Lifeline?Normal Human Bladder Epithelial Cells – Apex, when grown in UroLife? culture medium, provide an ideal culture model for the study of bladder biology.
Human Bladder Epithelial Cells -Apex are cryopreserved as primary cells, e.g., cells are isolated from the apex (neck or trigone) region of human bladder tissue and expanded in culture vessels before being harvested for cryopreservation.
Bladder Epithelial Cells – Apex are tested in complete UroLife? Medium to ensure proper growth and morphology.
Lifeline?Bladder Epithelial Cells need not be exposed to antimicrobials or phenol red when cultured in Lifeline?culture media. Lifeline?offers these traditional supplements, but they are not needed or recommended to achieve optimal cell performance in most research applications (an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.)
Normal Human Bladder Cells – Apex are tested for:
Proliferation and Morphology: Normal cell appearance for 15 population doublings
Viability: Minimum 70% viability when thawed from cryopreservation
Sterility: Negative for mycoplasma, Negative for bacterial and fungal growth
Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR